pLVX-SBP-mGFP-COL1A1
(Plasmid #110726)

• Purpose: 2nd generation lentiviral transfer plasmid. Expresses an engineered human procollagen type I alpha 1 with SBP and mGFP between the pro sequence and triple helical region
• Depositing Lab: David Stephens
• Publication: McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 10.1083/jcb.201806035.

Backbone

• Vector backbone: pLVX-Puro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8102
• Total vector size (bp): 13371
• Modifications to backbone: None other than cloning of insert
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pro-SBP-mGFP-COL1A1
• Alt name: mGFP-procollagen
• Species: H. sapiens (human), Synthetic
• Mutation: See attached PDF
• GenBank ID: AAB94054.3 P02452.5
• Entrez Gene: COL1A1 (a.k.a. CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mGFP
  • SBP: streptavidin binding peptide

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGC AAA TGG GCG GTA GGC GTG
• 3′ sequencing primer: GATGGCCACATCGGCAG

Resource Information

• Supplemental Documents:
  • Description of construct, changes in sequence and cloning methods.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See attached PDF for full descritions