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Addgene

p116 Mfz10a
(Plasmid #11070)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11070 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mfz10a
  • Alt name
    Mouse frizzled 10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2250
  • GenBank ID
    AF206322
  • Entrez Gene
    Fzd10 (a.k.a. Fz-10)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note in this version of pCS, the T7 transcription site functions poorly,and so the T3 transcription site would be a better choice to synthesize an antisense riboprobe.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p116 Mfz10a was a gift from Ramesh Shivdasani (Addgene plasmid # 11070 ; http://n2t.net/addgene:11070 ; RRID:Addgene_11070)

  • For your References section:

    Structure and expression of a novel frizzled gene isolated from the developing mouse gut. Malik TH, Shivdasani RA. Biochem J. 2000 Aug 1. 349 Pt 3():829-34. PubMed 10903145
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