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Addgene

pSICO-CPSF6-358-iRFP670
(Plasmid #110694)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110694 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSICO
  • Backbone manufacturer
    Tyler Jacks, Addgene plasmid #11578
  • Backbone size w/o insert (bp) 6855
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CPSF6-iRFP670
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2055
  • Mutation
    Truncation of CPSF6 isoform at amino acid 358
  • Entrez Gene
    CPSF6 (a.k.a. CFIM, CFIM68, CFIM72, HPBRII-4, HPBRII-7)
  • Tag / Fusion Protein
    • iRFP670 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSICO-CPSF6-358-iRFP670 was a gift from Zandrea Ambrose (Addgene plasmid # 110694 ; http://n2t.net/addgene:110694 ; RRID:Addgene_110694)

  • For your References section:

    Truncated CPSF6 forms higher order complexes that bind and disrupt HIV-1 capsid. Ning J, Zhong Z, Fischer DK, Harris G, Watkins SC, Ambrose Z, Zhang P. J Virol. 2018 Apr 11. pii: JVI.00368-18. doi: 10.1128/JVI.00368-18. 10.1128/JVI.00368-18 PubMed 29643241
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