pSICO-CPSF6-358-eGFP
(Plasmid #110693)

• Purpose: Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP
• Depositing Lab: Zandrea Ambrose
• Publication: Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18. doi: 10.1128/JVI.00368-18.

Backbone

• Vector backbone: pSICO
• Backbone manufacturer: Tyler Jacks, Addgene plasmid #11578
• Backbone size w/o insert (bp): 6852
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CPSF6-eGFP
• Insert Size (bp): 1839
• Mutation: Truncation of CPSF6 isoform 2 at amino acid 358
• Entrez Gene: CPSF6 (a.k.a. CFIM, CFIM68, CFIM72, HPBRII-4, HPBRII-7)
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry