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Addgene

pcDNA3-PBZ-mRuby2
(Plasmid #110650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-mRuby2
  • Backbone manufacturer
    Michael Lin, Addgene plasmid #40260
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PBZ domain from APLF, amino acids 371-451
  • Alt name
    Aprataxin and PNK-like factor
  • Species
    H. sapiens (human)
  • Entrez Gene
    APLF (a.k.a. APFL, C2orf13, PALF, Xip1, ZCCHH1)
  • Tag / Fusion Protein
    • mRuby2 (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-PBZ-mRuby2 was a gift from Chris Lord (Addgene plasmid # 110650 ; http://n2t.net/addgene:110650 ; RRID:Addgene_110650)

  • For your References section:

    Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets. Krastev DB, Pettitt SJ, Campbell J, Song F, Tanos BE, Stoynov SS, Ashworth A, Lord CJ. Nat Commun. 2018 May 22;9(1):2016. doi: 10.1038/s41467-018-04466-4. 10.1038/s41467-018-04466-4 PubMed 29789535
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