p176 Grg-1s Myc His
(Plasmid #11065)

• Depositing Lab: Ramesh Shivdasani
• Publication: Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-40.

Backbone

• Vector backbone: pcDNA3.1/myc-His B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Grg1-S
• Alt name: Groucho
• Alt name: Grg1 short
• Species: M. musculus (mouse)
• Insert Size (bp): 600
• Entrez Gene: Tle1 (a.k.a. C230057C06Rik, Estm14, Grg-1, Grg1, Tle4l)
• Tags / Fusion Proteins:
  • Myc (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry