pBiFC-PBZ-4A-VN
(Plasmid #110647)

• Purpose: expresses a PAR-binding (PBZ) domain fused to venus N-terminus, contains point mutations that abolish PAR binding
• Depositing Lab: Chris Lord
• Publication: Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi: 10.1038/s41467-018-04466-4.

Backbone

• Vector backbone: pBiFC-VN173
• Backbone manufacturer: Chang-Deng Hu, Addgene plasmid #22010
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PBZ domain from APLF, amino acids 371-451
• Alt name: Aprataxin and PNK-like factor
• Species: H. sapiens (human)
• Mutation: C379A, C385A, C421A and C427A
• Entrez Gene: APLF (a.k.a. APFL, C2orf13, PALF, Xip1, ZCCHH1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • DDK (N terminal on insert)
  • VN (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pBiFC-PBZ-4A-VN.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry