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Addgene

pMXs_FLAG-FSF1
(Plasmid #110641)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110641 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXs-IRES-Blasticidin
  • Backbone manufacturer
    Cell Biolabs, Inc.
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FSF1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1086
  • Mutation
    codon-optimized cDNA (no amino acid mutations)
  • Entrez Gene
    FSF1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI - FLAG tag - EcoRI - gene (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer GTGGACCATCCTCTAG
  • 3′ sequencing primer GACGGCAATATGGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs_FLAG-FSF1 was a gift from David Sabatini (Addgene plasmid # 110641 ; http://n2t.net/addgene:110641 ; RRID:Addgene_110641)

  • For your References section:

    SFXN1 is a mitochondrial serine transporter required for one-carbon metabolism. Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, Chan SH, Lewis CA, Wang T, Keys HR, Guo YE, Sabatini DM. Science. 2018 Nov 16;362(6416). pii: 362/6416/eaat9528. doi: 10.1126/science.aat9528. 10.1126/science.aat9528 PubMed 30442778
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