pMXs_SFXN5
(Plasmid
#110640)
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PurposeRetroviral expression of SFXN5 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110640 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs-IRES-Blasticidin
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Backbone manufacturerCell Biolabs, Inc.
- Backbone size w/o insert (bp) 5600
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSFXN5
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SpeciesH. sapiens (human)
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Insert Size (bp)1176
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Entrez GeneSFXN5 (a.k.a. BBG-TCC, MGC120413, MGC120415)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer GTGGACCATCCTCTAG
- 3′ sequencing primer GACGGCAATATGGTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs_SFXN5 was a gift from David Sabatini (Addgene plasmid # 110640 ; http://n2t.net/addgene:110640 ; RRID:Addgene_110640) -
For your References section:
SFXN1 is a mitochondrial serine transporter required for one-carbon metabolism. Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, Chan SH, Lewis CA, Wang T, Keys HR, Guo YE, Sabatini DM. Science. 2018 Nov 16;362(6416). pii: 362/6416/eaat9528. doi: 10.1126/science.aat9528. 10.1126/science.aat9528 PubMed 30442778