cfSBFP2-N
(Plasmid
#110633)
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PurposeCys-free blue fluorescent protein for extracellular imaging. SBFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SBFP2.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110633 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneEGFP-N1
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Backbone manufacturerClontech
- Total vector size (bp) 4754
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecfSBFP2
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SpeciesSynthetic
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Insert Size (bp)720
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MutationCys48Ser/Cys70Met
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GenBank IDEF030495.1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that although the sequencing software identifies the protein as a mEmerald variant, we have confirmed the the sequence is cfSBFP2 (C48S/C70M).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cfSBFP2-N was a gift from Ikuo Wada (Addgene plasmid # 110633 ; http://n2t.net/addgene:110633 ; RRID:Addgene_110633)