cfSYFP2-N
(Plasmid
#110631)
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PurposeCys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110631 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSYFP2-N
- Total vector size (bp) 4733
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecfSYFP2-N
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SpeciesSynthetic
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Insert Size (bp)720
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MutationCys48Ser and Cys70/Met
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Tag
/ Fusion Protein
- Multi-cloning sites (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that although the sequencing software identifies the protein as a mEmerald/Topaz YFP variant, we have confirmed the the sequence is SYFP2 (C48S/C70M).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cfSYFP2-N was a gift from Ikuo Wada (Addgene plasmid # 110631 ; http://n2t.net/addgene:110631 ; RRID:Addgene_110631)