p185 pK7-HDGF (GFP)
(Plasmid
#11058)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11058 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepK7
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHDGF
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SpeciesR. norvegicus (rat)
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Insert Size (bp)700
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Entrez GeneHdgf
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
*Note there is a Q104R mutation in the plasmid that is unlikely to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p185 pK7-HDGF (GFP) was a gift from Ramesh Shivdasani (Addgene plasmid # 11058 ; http://n2t.net/addgene:11058 ; RRID:Addgene_11058) -
For your References section:
Regulation of mammalian epithelial differentiation and intestine development by class I histone deacetylases. Tou L, Liu Q, Shivdasani RA. Mol Cell Biol. 2004 Apr . 24(8):3132-9. 10.1128/MCB.24.8.3132-3139.2004 PubMed 15060137