pAAV-IV-3xFLAG/HA/NBL10
(Plasmid #110568)

• Purpose: Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag
• Depositing Labs: Jeffrey Friedman, Alexander Nectow
• Publication: pAAV-IV-NBL10 (unpublished)

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Backbone

• Vector backbone: pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
• Backbone manufacturer: Karl Deisseroth
• Backbone size w/o insert (bp): 5484
• Total vector size (bp): 6900
• Vector type: Mammalian Expression, AAV, Cre/Lox, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3xFLAG/HA-tagged anti-GFP VHH
• Alt name: NBL10
• Species: Lama pacos (camelid)
• Insert Size (bp): 456
• Promoter: EF1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: Ef1a
• 3′ sequencing primer: WPRE

Resource Information

• A portion of this plasmid was derived from a plasmid made by: VHH is from: Rothbauer, U., Zolghadr, K., Tillib, S., Nowak, D., Schermelleh, L., Gahl, A., Backmann, N., Conrath, K., Muyldermans, S., Cardoso, M.C., and Leonhardt, H. (2006). Targeting and tracing antigens in live cells with fluorescent nano- bodies. Nat. Methods 3, 887–889.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry