EYFP-Talin1-FERM
(Plasmid
#110565)
-
Purposebacterial expresion of EYFP-tagged Talin 1
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 110565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET15b
-
Backbone manufacturerNovagen
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTalin 1
-
Alt nameTLN1
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1953
-
Entrez GeneTln1 (a.k.a. Tln)
- Promoter T7
-
Tag
/ Fusion Protein
- (His)6 (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
EYFP-Talin1-FERM was a gift from Tina Izard (Addgene plasmid # 110565 ; http://n2t.net/addgene:110565 ; RRID:Addgene_110565)