MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A
(Plasmid
#110555)
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PurposepFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110555 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFUSEss
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Backbone manufacturerInvivogen
- Backbone size w/o insert (bp) 3431
- Total vector size (bp) 4820
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-RD114 heavy chain
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Alt nameaRD114_CHIg-mIgG2A
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SpeciesRD114 retrovirus
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GenBank IDNCBI:txid11834
- Promoter EF-1 alpha core promoter
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Tag
/ Fusion Protein
- mIgG2A (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BlpI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A was a gift from Martin Pule (Addgene plasmid # 110555 ; http://n2t.net/addgene:110555 ; RRID:Addgene_110555) -
For your References section:
Generation of a neutralizing antibody against RD114-pseudotyped viral vectors. Mekkaoui L, Ferrari M, Mattiuzzo G, Ma B, Nannini F, Onuoha S, Kotsopoulou E, Takeuchi Y, Pule M. J Gen Virol. 2019 Nov 8. doi: 10.1099/jgv.0.001309. 10.1099/jgv.0.001309 PubMed 31702531
Map uploaded by the depositor.
