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Addgene

pFLAG-ZYG11A
(Plasmid #110551)

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Item Catalog # Description Quantity Price (USD)
Plasmid 110551 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZYG11A
  • Alt name
    ZYG-11A
  • Species
    H. sapiens (human)
  • Entrez Gene
    ZYG11A (a.k.a. ZYG11)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-ZYG11A was a gift from Edward Kipreos (Addgene plasmid # 110551 ; http://n2t.net/addgene:110551 ; RRID:Addgene_110551)

  • For your References section:

    The ubiquitin ligase CRL2ZYG11 targets cyclin B1 for degradation in a conserved pathway that facilitates mitotic slippage. Balachandran RS, Heighington CS, Starostina NG, Anderson JW, Owen DL, Vasudevan S, Kipreos ET. J Cell Biol. 2016 Oct 24;215(2):151-166. Epub 2016 Oct 17. 10.1083/jcb.201601083 PubMed 27810909
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