pFLAG-ZYG11B
(Plasmid #110550)

• Purpose: expresses a FLAG-tagged human ZYG11B protein in mamalian cells
• Depositing Lab: Edward Kipreos
• Publication: Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166. Epub 2016 Oct 17.

Backbone

• Vector backbone: pCMV-Tag2b
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZYG11B
• Alt name: ZYG-11B
• Species: H. sapiens (human)
• Entrez Gene: ZYG11B (a.k.a. ZYG11)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA