p182 pK7-HDAC1 mut (GFP)
(Plasmid #11055)

• Depositing Lab: Ramesh Shivdasani
• Publication: Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Backbone

• Vector backbone: pK7
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HDAC1
• Species: M. musculus (mouse)
• Insert Size (bp): 1300
• Mutation: Deletion of aa 1-56: deacetylase domain deletion
• Entrez Gene: Hdac1 (a.k.a. HD1, Hdac1-ps, MommeD5, RPD3)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing data finds T189A and D332G substitutions. These are not known to affect function.