pACT5FVMF(f)
(Plasmid #110549)

• Purpose: Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008
• Depositing Lab: David Russell
• Publication: Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10.1038/sj.mt.6300398. Epub 2008 Jan 22.

Backbone

• Vector backbone: pBluescript II KS+
• Backbone manufacturer: Stratagene, La Jolla, CA
• Backbone size w/o insert (bp): 5787
• Total vector size (bp): 10644
• Modifications to backbone: pACT5 contains a 795-bp spacer from intron 2 of the human HRPT gene, a minimal CMV promoter downstream of a tetracycline response element from pTREtight (BD Biosciences Clontech, Palo Alto, CA), the internal ribosome entry site element from pIRES2-EGFP (BD Biosciences Clontech), the firefly luciferase gene from pA2Rluc, a synthetic polyadenylation site and a bacterial backbone derived from pBluescript II KS+
• Vector type: qPCR control for titering foamy virus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP, FV LTRs
• Species: deleted foamy virus
• Insert Size (bp): 4857
• Promoter: promoter: MLV LTR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • Foamy Vector Genome Titer protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry