T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB
(Plasmid
#110547)
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PurposeCRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110547 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACYC
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Backbone manufacturerNovagen/Agilent
- Backbone size w/o insert (bp) 2412
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)OmniMax2
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedCas9
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SpeciesSynthetic; Streptococcus pyrogenes
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MutationD10A, H840A
- Promoter pBAD
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pIM2432
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB was a gift from Ichiro Matsumura (Addgene plasmid # 110547 ; http://n2t.net/addgene:110547 ; RRID:Addgene_110547)
