pTarKO
(Plasmid
#110540)
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Purpose(Empty Backbone) one-step modification of chromosomal locous using targocil/tarO gene in Staphylococcus aureus
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110540 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTarKO
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Backbone manufacturer4951
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Vector typeSynthetic Biology ; targeting vector for locus of staphylococcus aureus geneome
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Selectable markerschloramphenicol for staphylococcus aureus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTarKO was a gift from Suzanne Walker (Addgene plasmid # 110540 ; http://n2t.net/addgene:110540 ; RRID:Addgene_110540) -
For your References section:
Antibiotic Combinations That Enable One-Step, Targeted Mutagenesis of Chromosomal Genes. Lee W, Do T, Zhang G, Kahne D, Meredith TC, Walker S. ACS Infect Dis. 2018 Mar 23. doi: 10.1021/acsinfecdis.8b00017. 10.1021/acsinfecdis.8b00017 PubMed 29534563
Map uploaded by the depositor.
