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Addgene

pRS315Nab3D191stop
(Plasmid #110537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NAB3
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    C-terminal deltion of 191 amino acids
  • Entrez Gene
    NAB3 (a.k.a. YPL190C, HMD1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS315Nab3D191stop was a gift from Daniel Reines (Addgene plasmid # 110537 ; http://n2t.net/addgene:110537 ; RRID:Addgene_110537)

  • For your References section:

    The hnRNP-like Nab3 termination factor can employ heterologous prion-like domains in place of its own essential low complexity domain. Loya TJ, O'Rourke TW, Reines D. PLoS One. 2017 Oct 12;12(10):e0186187. doi: 10.1371/journal.pone.0186187. eCollection 2017. 10.1371/journal.pone.0186187 PubMed 29023495
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