pRS315Nab3
(Plasmid
#110532)
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PurposeExpresses yeast NAB3 using the endogenous promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 110532 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS315
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNAB3
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2932
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Entrez GeneNAB3 (a.k.a. YPL190C, HMD1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS315Nab3 was a gift from Daniel Reines (Addgene plasmid # 110532 ; http://n2t.net/addgene:110532 ; RRID:Addgene_110532) -
For your References section:
A genetic screen for terminator function in yeast identifies a role for a new functional domain in termination factor Nab3. Loya TJ, O'Rourke TW, Reines D. Nucleic Acids Res. 2012 Aug;40(15):7476-91. doi: 10.1093/nar/gks377. Epub 2012 May 7. 10.1093/nar/gks377 PubMed 22564898