p180 pCIG-HDAC1 mut
(Plasmid
#11053)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11053 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCIG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHDAC1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1300
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MutationDeletion of aa 1-56: deacetylase domain deletion
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Entrez GeneHdac1 (a.k.a. HD1, Hdac1-ps, MommeD5, RPD3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pCIG drives simultaneous expression of GFP and inserted genes by virtue of an internal ribosome entry site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p180 pCIG-HDAC1 mut was a gift from Ramesh Shivdasani (Addgene plasmid # 11053 ; http://n2t.net/addgene:11053 ; RRID:Addgene_11053) -
For your References section:
Regulation of mammalian epithelial differentiation and intestine development by class I histone deacetylases. Tou L, Liu Q, Shivdasani RA. Mol Cell Biol. 2004 Apr . 24(8):3132-9. 10.1128/MCB.24.8.3132-3139.2004 PubMed 15060137