pcDNA3 HRPT2 L64P
(Plasmid #11052)

• Depositing Lab: Matthew Meyerson
• Publication: Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HRPT2 L64P
• Alt name: HRPT2
• Alt name: parafibromin
• Species: H. sapiens (human)
• Insert Size (bp): 1596
• Mutation: L64P
• Entrez Gene: CDC73 (a.k.a. C1orf28, FIHP, HPTJT, HRPT1, HRPT2, HYX)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Author has deposited insert sequence. Click on "sequence" to view. Used plasmid 11048 as a template for mutagenesis using the following primers: 5' G GAT TCC ATT TTA TTT CTA CCT AAT AAC GTG CAC CTT TCT C 3' and 5' G AGA AAG GAG CAC GTT ATT AGG TAG AAA TAA AAT GGA ATC C 3'