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Addgene

ECFP-Rab5C-S35N
(Plasmid #110504)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110504 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab5C-S35N
  • Species
    H. sapiens (human)
  • Mutation
    S35N
  • Entrez Gene
    RAB5C (a.k.a. L1880, RAB5CL, RAB5L, RABL)
  • Tag / Fusion Protein
    • ECFP (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ECFP-Rab5C-S35N was a gift from Terence Dermody & Bernardo Mainou (Addgene plasmid # 110504 ; http://n2t.net/addgene:110504 ; RRID:Addgene_110504)

  • For your References section:

    Transport to late endosomes is required for efficient reovirus infection. Mainou BA, Dermody TS. J Virol. 2012 Aug;86(16):8346-58. doi: 10.1128/JVI.00100-12. Epub 2012 Jun 6. 10.1128/JVI.00100-12 PubMed 22674975
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