EGFP-Rab13-T22N
(Plasmid
#110501)
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PurposeDominant Negative
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110501 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRab13-T22N
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SpeciesH. sapiens (human)
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MutationT22N
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Entrez GeneRAB13 (a.k.a. GIG4)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-Rab13-T22N was a gift from Bernardo Mainou (Addgene plasmid # 110501 ; http://n2t.net/addgene:110501 ; RRID:Addgene_110501) -
For your References section:
Transport to late endosomes is required for efficient reovirus infection. Mainou BA, Dermody TS. J Virol. 2012 Aug;86(16):8346-58. doi: 10.1128/JVI.00100-12. Epub 2012 Jun 6. 10.1128/JVI.00100-12 PubMed 22674975