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Addgene

Src-EGFP
(Plasmid #110496)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110496 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-Src
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Src (a.k.a. pp60c-src)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Src-EGFP was a gift from Terence Dermody & Bernardo Mainou (Addgene plasmid # 110496 ; http://n2t.net/addgene:110496 ; RRID:Addgene_110496)

  • For your References section:

    Src kinase mediates productive endocytic sorting of reovirus during cell entry. Mainou BA, Dermody TS. J Virol. 2011 Apr;85(7):3203-13. doi: 10.1128/JVI.02056-10. Epub 2011 Jan 19. 10.1128/JVI.02056-10 PubMed 21248042
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