pSKC12 - DelQ
(Plasmid #110493)

• Purpose: Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.
• Depositing Lab: Shankar Balasubramanian
• Publication: Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi: 10.1038/nchembio864. Epub 2007 Feb 25.

Backbone

• Vector backbone: pUC18
• Total vector size (bp): 2680
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NRAS 5'- DelQ (bp 30-254)
• Species: H. sapiens (human)
• Insert Size (bp): 224
• Mutation: Deletion of the first 29 base pairs of the NRAS 5′ UTR (sequence comprising the RNA G-quadruplex-forming sequence)
• GenBank ID: AM489487
• Entrez Gene: NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
• Promoter: T7
• Tag / Fusion Protein:
  • Firefly luciferase (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13-fwd
• 3′ sequencing primer: M13-rev

Resource Information

• Supplemental Documents:
  • pSKC12 - DelQ.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry