Skip to main content
Addgene

pSKC11 - UTRQ
(Plasmid #110490)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC18
  • Total vector size (bp) 2680
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NRAS 5'-UTR (bp 1-254)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    254
  • GenBank ID
    AM489486
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
  • Promoter T7
  • Tag / Fusion Protein
    • Firefly luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13-fwd
  • 3′ sequencing primer M13-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSKC11 - UTRQ was a gift from Shankar Balasubramanian (Addgene plasmid # 110490 ; http://n2t.net/addgene:110490 ; RRID:Addgene_110490)

  • For your References section:

    An RNA G-quadruplex in the 5' UTR of the NRAS proto-oncogene modulates translation. Kumari S, Bugaut A, Huppert JL, Balasubramanian S. Nat Chem Biol. 2007 Apr;3(4):218-21. doi: 10.1038/nchembio864. Epub 2007 Feb 25. 10.1038/nchembio864 PubMed 17322877
Related items:
Commonly requested with: