pUltra-Exon1Q145-Myc-B
(Plasmid #110489)

• Purpose: Lentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)
• Depositing Lab: Baoji Xu
• Publication: Xu et al PLoS One. 2017 May 11;12(5):e0177610. doi: 10.1371/journal.pone.0177610. eCollection 2017.

Backbone

• Vector backbone: pUltra
• Backbone manufacturer: Malcolm Moore, Addgene plasmid #24129
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens huntingtin
• Alt name: NM_002111
• Species: H. sapiens (human)
• Mutation: partial long 3'UTR wild type huntingtin exon1 fragment with 145 repeats of Q
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Promoter: UbC
• Tag / Fusion Protein:
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry