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Addgene

pUltra-Exon1Q23-Myc-B
(Plasmid #110488)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110488 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUltra
  • Backbone manufacturer
    Malcolm Moore, Addgene plasmid #24129
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Homo sapiens huntingtin
  • Alt name
    NM_002111
  • Species
    H. sapiens (human)
  • Mutation
    short 3'UTR mutant huntingtin exon1 fragment with 23 repeats of Q
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Promoter UbC
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUltra-Exon1Q23-Myc-B was a gift from Baoji Xu (Addgene plasmid # 110488 ; http://n2t.net/addgene:110488 ; RRID:Addgene_110488)

  • For your References section:

    Distinct cellular toxicity of two mutant huntingtin mRNA variants due to translation regulation. Xu H, An JJ, Xu B. PLoS One. 2017 May 11;12(5):e0177610. doi: 10.1371/journal.pone.0177610. eCollection 2017. 10.1371/journal.pone.0177610 PubMed 28494017
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