Ultra-Exon1Q23-Myc-A
(Plasmid
#110486)
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PurposeLentiviral vector to express wild-type N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged wild type huntingtin exon1 fragment with 23 repeats of Q)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUltra
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Backbone manufacturerMalcolm Moore, Addgene plasmid #24129
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHomo sapiens huntingtin
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Alt nameNM_002111
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SpeciesH. sapiens (human)
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MutationShort 3'UTR wild type huntingtin exon1 fragment with 23 repeats of Q
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Entrez GeneHTT (a.k.a. HD, IT15, LOMARS)
- Promoter UbC
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ultra-Exon1Q23-Myc-A was a gift from Baoji Xu (Addgene plasmid # 110486 ; http://n2t.net/addgene:110486 ; RRID:Addgene_110486) -
For your References section:
Distinct cellular toxicity of two mutant huntingtin mRNA variants due to translation regulation. Xu H, An JJ, Xu B. PLoS One. 2017 May 11;12(5):e0177610. doi: 10.1371/journal.pone.0177610. eCollection 2017. 10.1371/journal.pone.0177610 PubMed 28494017