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p077 pCS xTRPS1 (M)
(Plasmid #11047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS105
  • Backbone size w/o insert (bp) 4133
  • Vector type
    mammalian, avian, and zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRPS1 mut
  • Alt name
    TRPS1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    3800
  • Mutation
    Two Cys residues in the GATA-type zinc finger were mutated to yield the sequence FGANAL instead of FCANCL.
  • Entrez Gene
    trps1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI? (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3 or M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Can be expressed in cos cells, does not give correct sized product by in-vitro translation, and cannot be expressed in xenopus embryos.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p077 pCS xTRPS1 (M) was a gift from Ramesh Shivdasani (Addgene plasmid # 11047 ; http://n2t.net/addgene:11047 ; RRID:Addgene_11047)

  • For your References section:

    Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1. Malik TH, Shoichet SA, Latham P, Kroll TG, Peters LL, Shivdasani RA. EMBO J. 2001 Apr 2. 20(7):1715-25. 10.1093/emboj/20.7.1715 PubMed 11285235
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