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Addgene

pBMN-2xFKBP-mEGFP-NDP52
(Plasmid #110465)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110465 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBMN-mEGFP-C1
  • Backbone size w/o insert (bp) 5747
  • Total vector size (bp) 7070
  • Vector type
    Mammalian Expression, Retroviral ; retrovirus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NDP52
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1338
  • GenBank ID
    NM_005831.4
  • Entrez Gene
    CALCOCO2 (a.k.a. NDP52)
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGAAGCGCGATCACATGG
  • 3′ sequencing primer AGGTGGGGTCTTTCATTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN-2xFKBP-mEGFP-NDP52 was a gift from Richard Youle (Addgene plasmid # 110465 ; http://n2t.net/addgene:110465 ; RRID:Addgene_110465)

  • For your References section:

    The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Lazarou M, Sliter DA, Kane LA, Sarraf SA, Wang C, Burman JL, Sideris DP, Fogel AI, Youle RJ. Nature. 2015 Aug 20;524(7565):309-14. doi: 10.1038/nature14893. Epub 2015 Aug 12. 10.1038/nature14893 PubMed 26266977
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