p075 pCS xTRPS1 delN delC119 (M)
(Plasmid #11045)

• Depositing Lab: Ramesh Shivdasani
• Publication: Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Backbone

• Vector backbone: pCS105
• Backbone size w/o insert (bp): 4133
• Vector type: xenopus, mammalian, avian, and zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRPS1 delN delC119 mut
• Alt name: TRPS1
• Species: X. laevis (frog)
• Insert Size (bp): 1050
• Mutation: aa806-1153 of xTRPS1. Two Cys residues in the GATA-type zinc finger were mutated to yield the sequence FGANAL instead of FCANCL.
• Entrez Gene: trps1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3 or M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Can be expressed in cos cells, gives the correct sized product by in-vitro tranlation and can be expressed in xenopus embryos.