Skip to main content
Addgene

p073 pCS xGATA4tr + xTRPS1(C119)
(Plasmid #11044)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCS105
  • Backbone size w/o insert (bp) 4133
  • Vector type
    xenopus, mammalian, avian, and zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GATA4tr + TRPS1(C119)
  • Alt name
    GATA4
  • Alt name
    TRPS1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1180
  • Mutation
    Fusion construct of XGATA4 (amino acids 1-274) with the terminal 119 amino acids (1153-1272) of XTRPS1
  • Entrez Gene
    trps1
  • Entrez Gene
    gata4-b (a.k.a. gata-4, gata4, xgata4)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Does not bind DNA as part of the basic domain is missing. This is a fusion construct of XGATA4 with the repressive domain of XTRPS1 which encodes two Ikaros type zinc fingers.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p073 pCS xGATA4tr + xTRPS1(C119) was a gift from Ramesh Shivdasani (Addgene plasmid # 11044 ; http://n2t.net/addgene:11044 ; RRID:Addgene_11044)

  • For your References section:

    Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1. Malik TH, Shoichet SA, Latham P, Kroll TG, Peters LL, Shivdasani RA. EMBO J. 2001 Apr 2. 20(7):1715-25. 10.1093/emboj/20.7.1715 PubMed 11285235
Related items: