p072 pCS xGATA4 + xTRPS1(C119)
(Plasmid
#11043)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS105
- Backbone size w/o insert (bp) 4133
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Vector typexenopus, mammalian, avian, and zebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 or M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Binds DNA. Fusion construct of XGATA4 with the repressive domain of XTRPS1, which encodes two Ikaros type zinc fingers.
Despite slight sequence variation, the gene is still GATA4. Xenopus being a tetraploid species and one that is not inbred, there is minor drift among alleles and our clone was probably derived from such a variant allele. The homology remains sound and the function is intact.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p072 pCS xGATA4 + xTRPS1(C119) was a gift from Ramesh Shivdasani (Addgene plasmid # 11043 ; http://n2t.net/addgene:11043 ; RRID:Addgene_11043) -
For your References section:
Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1. Malik TH, Shoichet SA, Latham P, Kroll TG, Peters LL, Shivdasani RA. EMBO J. 2001 Apr 2. 20(7):1715-25. 10.1093/emboj/20.7.1715 PubMed 11285235