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Addgene

pX330.orup
(Plasmid #110404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330-U6-Chimeric_BB-CBh-hSpCas9
  • Backbone manufacturer
    #42230
  • Backbone size (bp) 8506
  • Modifications to backbone
    NotI site cleaved and filled, SV40-Puro cassete from pBABE.puro (Addgene #1764 from Dr. Hartmut Land, Dr. Jay Morgenstern, Dr. Bob Weinberg) with BamHI (overhang removed) and XbaI (overhang filled).
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter CBh
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pX330 with puromycin resistance

For plasmid usage, please see the associated publication (Cong et al. Science. 2013, PMID: 23287718), as well as Ran et al. Nat Protoc. 2013, PMID: 24157548.

For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330.orup was a gift from Sandra Martha Gomes Dias (Addgene plasmid # 110404 ; http://n2t.net/addgene:110404 ; RRID:Addgene_110404)
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