Skip to main content
Addgene

p068 pCS mTRPS1 (M)
(Plasmid #11040)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11040 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCS105
  • Backbone size w/o insert (bp) 4133
  • Vector type
    xenopus, mammalian, avian, and zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRPS1 mut
  • Alt name
    TRPS1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4300
  • Mutation
    Two Cys residues at positions 917 and 920 in the GATA-type zinc finger were mutated to yield the sequence VGNAGG instead of VCNACG.
  • Entrez Gene
    Trps1 (a.k.a. AI115454, AI447310, D15Ertd586e)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3 or M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full coding + UTR of mTRPS1. Can be expressed in cos cell, xenopus embryos, and in vitro translation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p068 pCS mTRPS1 (M) was a gift from Ramesh Shivdasani (Addgene plasmid # 11040 ; http://n2t.net/addgene:11040 ; RRID:Addgene_11040)

  • For your References section:

    Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1. Malik TH, Shoichet SA, Latham P, Kroll TG, Peters LL, Shivdasani RA. EMBO J. 2001 Apr 2. 20(7):1715-25. 10.1093/emboj/20.7.1715 PubMed 11285235
Related items:
Commonly requested with: