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Addgene

pcDNA5/FRT/TO/Flag-tGFP
(Plasmid #110375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO/FLAG
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5150
  • Total vector size (bp) 5852
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    turboGFP
  • Alt name
    Improved CopGFP
  • Alt name
    evrogen turbo GFP
  • Species
    Pontellina plumata
  • Insert Size (bp)
    696
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV Forward 5'-CGCAAATGGGCGGTAGGCGTG-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO/Flag-tGFP was a gift from Sandra Martha Gomes Dias (Addgene plasmid # 110375 ; http://n2t.net/addgene:110375 ; RRID:Addgene_110375)
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