pcDNA5/FRT/TO/Flag-mKO2-HuR.DelHNS
(Plasmid #110373)

• Purpose: Mammalian expression of mKO2-HuR (DelHNS) with FLAG tag, Flp-In T-Rex system
• Depositing Lab: Sandra Martha Gomes Dias
• Publication: Mammalian expression plasmids based on pcDNA5/FRT/TO family (unpublished)

Backbone

• Vector backbone: pcDNA5/FRT/TO/FLAG-mKO2
• Backbone manufacturer: #110371
• Backbone size w/o insert (bp): 5834
• Total vector size (bp): 6688
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ELAV like RNA binding protein 1
• Alt name: ELAVL1
• Alt name: HuR
• Species: H. sapiens (human)
• Insert Size (bp): 981
• Mutation: HNS (HuR nuclear-cytoplasmic shuttling sequence) deleted, 205-237, replaced by lisine-leucine (KL), HindIII site
• GenBank ID: 1994 NM_001419
• Entrez Gene: ELAVL1 (a.k.a. ELAV1, HUR, Hua, MelG)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • mKO2 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV Forward 5'-CGCAAATGGGCGGTAGGCGTG-3'

Resource Information

• Supplemental Documents:
  • pD145-pcDNA5_FRT_TO_Flag_mKO2_HuR.DelHNS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert created by joining regions before (Addgene #110395) and after (Addgene #110395) HuR HNS by HindIII restriction site