p065 pCS mTRPS1 P2
(Plasmid
#11037)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11037 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS105
- Backbone size w/o insert (bp) 4133
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Vector typexenopus, mammalian, avian, and zebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRPS1 P2
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Alt nameTRPS1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1833
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MutationTruncation at aa611 to mimic R611X mutation found in patients.
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Entrez GeneTrps1 (a.k.a. AI115454, AI447310, D15Ertd586e)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 or M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see Momeni et al (Nature Genetics 24: 71-74) to learn more about the patient mutation.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p065 pCS mTRPS1 P2 was a gift from Ramesh Shivdasani (Addgene plasmid # 11037 ; http://n2t.net/addgene:11037 ; RRID:Addgene_11037) -
For your References section:
Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1. Malik TH, Shoichet SA, Latham P, Kroll TG, Peters LL, Shivdasani RA. EMBO J. 2001 Apr 2. 20(7):1715-25. 10.1093/emboj/20.7.1715 PubMed 11285235