p063 pCS xTRPS1 delta N (M)
(Plasmid
#11036)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11036 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS105
- Backbone size w/o insert (bp) 4133
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Vector typemammalian, avian, and zebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRPS1 deltaN mut
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Alt nameTRPS1
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SpeciesX. laevis (frog)
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Insert Size (bp)1400
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Mutationaa806-1153 of xTRPS1. Two Cys residues in the GATA-type zinc finger were mutated to yield the sequence FGANAL instead of FCANCL.
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Entrez Genetrps1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 or M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p063 pCS xTRPS1 delta N (M) was a gift from Ramesh Shivdasani (Addgene plasmid # 11036 ; http://n2t.net/addgene:11036 ; RRID:Addgene_11036) -
For your References section:
Transcriptional repression and developmental functions of the atypical vertebrate GATA protein TRPS1. Malik TH, Shoichet SA, Latham P, Kroll TG, Peters LL, Shivdasani RA. EMBO J. 2001 Apr 2. 20(7):1715-25. 10.1093/emboj/20.7.1715 PubMed 11285235