pBABE.zeo/TR
(Plasmid #110358)

• Purpose: Mammalian retroviral expression of Tetracycline repressor with β-globin intron for doxycycline inducible systems
• Depositing Lab: Sandra Martha Gomes Dias
• Publication: Mammalian retroviral expression plasmids based on pBABE family (unpublished)

Backbone

• Vector backbone: pBABE-zeo (pBABE-bleo)
• Backbone manufacturer: #1766
• Backbone size w/o insert (bp): 4902
• Total vector size (bp): 6290
• Modifications to backbone: β-globin intron - TetR cassete obtained from pcDNA6/TR (Invitrogen)
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tetracycline repressor
• Alt name: TetR
• Species: Synthetic
• Insert Size (bp): 618
• Promoter: gag truncated

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pBABE 5' 5'-CTTTATCCAGCCCTCAC-3'

Resource Information

• Supplemental Documents:
  • pD176-pBABE.zeocin_TR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBABE-based γ-Retroviral transfer vector for cloning tetracycline repressor with SV40-driven zeocin resistance.

Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.