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pBABE.zeo/hKGA.S286A
(Plasmid #110357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 110357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-zeo (pBABE-bleo)
  • Backbone manufacturer
    #1766
  • Backbone size w/o insert (bp) 4902
  • Total vector size (bp) 6990
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KGA glutaminase
  • Alt name
    GLS (KGA Isoform)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2007
  • Mutation
    S286A
  • GenBank ID
    2744 NM_014905
  • Entrez Gene
    GLS (a.k.a. AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG, GLS1, KGA)
  • Promoter gag
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5' 5'-CTTTATCCAGCCCTCAC-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE.zeo/hKGA.S286A was a gift from Sandra Martha Gomes Dias (Addgene plasmid # 110357 ; http://n2t.net/addgene:110357 ; RRID:Addgene_110357)
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