pBABE.puro/TO/Flag/mKO2
(Plasmid #110353)

• Purpose: Doxycycline inducible mammalian retroviral expression of mKO2 with FLAG tag
• Depositing Lab: Sandra Martha Gomes Dias
• Publication: Mammalian retroviral expression plasmids based on pBABE family (unpublished)

Backbone

• Vector backbone: pBABE-puro
• Backbone manufacturer: Plasmid #1764
• Backbone size w/o insert (bp): 5177
• Total vector size (bp): 6051
• Modifications to backbone: Sequence obtained from pcDNA5/FRT/TO/Flag-mKO2 (Addgene #110371) using BglII-XhoI enzymes, containing 1xTO/Flag and mKO2
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: monomeric Kusabira Orange 2
• Alt name: mKO2
• Species: Synthetic
• Insert Size (bp): 651
• GenBank ID:
• Promoter: gag
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: pBABE 5' 5'-CTTTATCCAGCCCTCAC-3'

Resource Information

• Supplemental Documents:
  • pD170-pBABE.puro_TO_Flag_mKO2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.