p064 pCS mTRPS1 P1
(Plasmid #11035)

• Depositing Lab: Ramesh Shivdasani
• Publication: Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Backbone

• Vector backbone: pCS105
• Backbone size w/o insert (bp): 4133
• Vector type: xenopus, mammalian, avian, and zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRPS1 P1
• Alt name: TRPS1
• Species: M. musculus (mouse)
• Insert Size (bp): 1000
• Mutation: Truncation at aa338 to mimic C338X mutation found in patients.
• Entrez Gene: Trps1 (a.k.a. AI115454, AI447310, D15Ertd586e)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3 or M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see Momeni et al (Nature Genetics 24: 71-74) to learn more about the patient mutation.