pQC hGAB.wt V5-His IRES G418
(Plasmid #110333)

• Purpose: Mammalian retroviral expression of glutaminase 2 isoform GAB (wild type) with V5 and 6xHis tags
• Depositing Lab: Sandra Martha Gomes Dias
• Publication: Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 10.1038/s41388-019-1007-z. Epub 2019 Sep 20.

Backbone

• Vector backbone: pQC MCS IRES G418
• Backbone manufacturer: #110344
• Backbone size w/o insert (bp): 7423
• Total vector size (bp): 9375
• Modifications to backbone: V5 and 6xHis tags at insert C-Terminal
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GLS2 Glutaminase 2
• Alt name: GAB isoform
• Species: H. sapiens (human)
• Insert Size (bp): 1809
• GenBank ID: 27165 NM_013267
• Entrez Gene: GLS2 (a.k.a. GA, GLS, LGA, hLGA)
• Promoter: CMV
• Tags / Fusion Proteins:
  • V5 (C terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: PmeI (destroyed during cloning)
• 5′ sequencing primer: pQC Seq Fow 5'-ACGCCATCCACGCTGTTTTGACCT-3'
• 3′ sequencing primer: pQC Seq Rev 5'-AAGCGGCTTCGGCCAGTAACGTTA-3'

Resource Information

• Supplemental Documents:
  • pD017-pQC hGAB.wt V5-His IRES G418.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pQC-based γ-Retroviral transfer vector with GAB.wt-V5-6xHis. IRES-driven Geneticin selection.

Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.