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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS105
- Backbone size w/o insert (bp) 4133
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Vector typeMammalian Expression ; Xenopus, Avian, and Zebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTcf-4
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Alt nameTcf7l2
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SpeciesM. musculus (mouse)
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Entrez GeneTcf7l2 (a.k.a. TCF4B, TCF4E, Tcf-4, Tcf4)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 or M13 reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Positive clone encoding mTcf4 obtained from screening a mouse embryonic gut cDNA library with degenerate oligos complementary to the highly conserved HMG box of Tcf/LEF proteins.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p043 mTcf-4 B was a gift from Ramesh Shivdasani (Addgene plasmid # 11031 ; http://n2t.net/addgene:11031 ; RRID:Addgene_11031) -
For your References section:
A possible role for the high mobility group box transcription factor Tcf-4 in vertebrate gut epithelial cell differentiation. Lee YJ, Swencki B, Shoichet S, Shivdasani RA. J Biol Chem. 1999 Jan 15. 274(3):1566-72. 10.1074/jbc.274.3.1566 PubMed 9880534